Facts About attune flow cytometer Revealed
Facts About attune flow cytometer Revealed
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Info-enriched characterization of mobile populations. Morphological information and facts from photos can insert to your richness of flow cytometry details. By way of example, the figure reveals an if not traditional apoptosis assay using Annexin V and PI, introducing mobile imaging to characterize cells in Every population to expose morphologically unique options. These insights couldn't happen to be acquired from multiplex staining alone.
As samples are obtained over the Attune CytPix Flow Cytometer, the higher-speed brightfield digicam captures and shops photographs of detected situations in a fee up to six,000 images for every 2nd, depending on the flow price and image dimensions. For higher adaptability, Attune Cytometric Application allows you to regulate the impression seize frequency as needed and select a specific gate for graphic seize, if essential.
Combine acoustic concentrating technological know-how with superior-parameter Assessment for versatile spectral unmixing and standard workflows.
Find new insights into your experimental knowledge by visualizing populations using merged fluorescence and morphology data. Our program interface wizards permit downsampling, concatenation (merged data files) and dimensionality resources that are crafted in the computer software for an intuitive, streamlined details Investigation expertise.
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On top of that, it's illuminating to check out the amount of doublets slip as a result of regular singlet gates, and great to validate the position of unforeseen double-beneficial situations in my Assessment."
Pair your Attune flow cytometer with an autosampler for amplified workflow effectiveness, and insert robotic integration for optimum throughput.
Our researchers will structure panels for you as again and again you would like—totally free, no merchandise obtain essential.
Early indications of this contamination had to begin with been dismissed as debris. Backgating to find out new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also means that you can use morphological capabilities to find interesting subpopulations that may not be obvious from flow cytometry facts by yourself.
Users can check out celebration pictures in true-time to confirm sample quality, optimize protocols, and history the morphology of each and every cell inhabitants recognized for Investigation. Imaging seize also Rewards from acoustic concentrating, which really helps to place the cells to ensure that a pointy, centered image check here is obtained.
AI-pushed automated picture Examination Using the Attune Cytometric Program translates party attributes into unique morphology parameters that can be combined with common fluorescence and scatter parameters to reveal very reproducible populace modifications and exclusive subsets.
We Beforehand shown the strength of imaging Vehicle-T/Ramos mobile interactions. Allow’s check out just the inhabitants of best desire, the double optimistic activities, To find out more. We are able to now use prolonged morphological parameters (listed here, circularity and skewness of depth) to even more analyze the capabilities of such populations and refine gating on these events, expanding facts robustness.
Leverage the strength of State-of-the-art technological know-how and innovation to accelerate your discoveries. Have confidence in while in the Attune Flow Cytometer family members to deliver the dependability, pace, and precision that established your work aside.
No clean/no lyse leukocyte Assessment with imaging. Cells ended up obtained from 24-hour-outdated blood by dilution in 1 mM EDTA ( Skilled UX/UI allows end users to prepare AI models for expanded applications. (A) The “Educate Product” element gives annotation instruments and genuine-time product training precision.
Gating on these prolonged parameters enables you to quickly and precisely determine populations of curiosity and confirm gating strategy with little or no guide overview. The Attune program features access to point out-of-the-art device Finding out Evaluation equipment inside an expertly created user interface, which makes it exceptionally easy to use.
Since the mobile line experienced Formerly been analyzed about the Attune CytPix Flow Cytometer, the scientists went back again to the pictures and were being ready to document the microbial an infection at the very least 5 days earlier.
Agilent Price Assure guarantees you no less than a decade of instrument use with the date you buy. Usually, We are going to credit score you Along with the residual worth of the system towards an upgraded model.
What gave the impression to be a homogeneous inhabitants is definitely far more varied—an Perception that ought to be regarded as when interpreting final results.